Targeted Sequencing of the Short Arm of Chromosome 6V of a Wheat Relative Haynaldia villosa for Marker Development and Gene Mining

The short arm of chromosome 6V (6VS) Haynaldia villosa has been used in wheat breeding programs to introduce Pm21 resistance gene against powdery mildew (Pm) and some other genes. In this study, 6VS was flow-sorted from wheat-H. ditelosomic addition line Dt6VS sequenced by Illumina technology. An assembly 230.39 Mb built with contig N50 9.788 bp. total, 3.276 high-confidence genes were annotated supported RNA sequencing data. Repetitive elements represented 74.91% the assembly. homologous identified on group 6 six Triticeae species confirming their synteny relationships. Out 45 NB-ARC domain proteins 6VS, 15 upregulated might also be involved innate immunity H. Pm. High thousand grain weight (TGW) for 6VS/6AL translocation not attributable GW2-6V gene. Based intron size differences, 119 intron-target (IT) markers developed trace chromatins introduced into background. assembled genome sequence specific IT work will facilitate mining utilization agronomic important 6VS.